The article studies species spreading of order Leptosphaeria Ces. & De Not., ecology and founding of plant species Salix alba L., Tilia cordata Mill. and Picea pungens Engelm. in Kashkadarya oasis. It is defined that 3 species of fungi such as Leptosphaeria baggei (Auersw. & Niessl) Sacc., Leptosphaeria sp., Leptosphaeria pini (Cruchet) E. Müll. belonging to the genus Leptosphaeria Ces. & De Not. are found. It was observed that Leptosphaeria pini (Cruchet) E. Mull. which among the identified species are a new species for the mycobiota of Uzbekistan.