According to the latest statistical reports from the World Health Organization (WHO), 13% of the world's population is obese. The most effective therapeutic approach for treating obesity is the inhibition of pancreatic lipase (PL). In the process of researching new PL inhibitors, we screened the anti-lipase activity of intracellular metabolites of 122 endophytic fungi isolated from medicinal plants growing in Uzbekistan. The highest number of endophytes with a high degree of PL inhibitory activity was found in 18 extracts (70-87%). The data obtained showed that metabolites capable of inhibiting pancreatic lipase at the Xenical standard level (more than 70%) are synthesized by endophytes isolated from the medicinal plants Gingko biloba, Aloe vera, Viola odorata, Taraxacum officinale, Achillea millefolium, Achillea tomentosa, and Peganum harmala. Based on this, we can conclude that endophytic fungi are a potential source of PL inhibitors, which may lead to the creation of new valuable drugs for the treatment of obesity.